Ultrasensitive immunoassay based on a pseudobienzyme amplifying system of choline oxidase and luminol-reduced Pt@Au hybrid nanoflowers.
نویسندگان
چکیده
A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.
منابع مشابه
Direct Competitive Chemiluminescence Immunoassays Based on Gold Magnetic for Ultrasensitive Detection of Chloramphenicol
Chemiluminescent immunoassays (CLIA) based on gold magnetic nanoparticles (AuMNPs) were developed for rapid analysis of chloramphenicol (CAP) in milk sample. Anti-CAP antibodies were immobilized on the surfaces of Au-MNPs, luminol (Method I) and 2',6'DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'-NHS Ester, (NSP-DMAE-NHS, Method II) were exploited in competitive CLIA for CAP d...
متن کاملHemin/G-quadruplex simultaneously acts as NADH oxidase and HRP-mimicking DNAzyme for simple, sensitive pseudobienzyme electrochemical detection of thrombin.
For the first time, hemin/G-quadruplex was employed to simultaneously serve as NADH oxidase and an HRP-mimicking DNAzyme for constructing a simple and sensitive pseudobienzyme-amplifying electrochemical aptasensor for thrombin detection.
متن کاملHighly enhanced electrochemiluminescence based on synergetic catalysis effect of enzyme and Pd nanoparticles for ultrasensitive immunoassay.
An immunosensor based on the electrochemiluminescence (ECL) of luminol was proposed by coupling enzymatic reaction to in situ generate coreactant with Pd nanoparticles as catalyst for the ECL reaction, which was successfully applied for the ultrasensitive detection of α-1-fetoprotein with a low detection limit of 33 fg mL(-1).
متن کاملA Novel Choline Biosensor Based on Immobilization of Enzyme Choline Oxidase on the β-Ga2O3 Nanowires Modified Working Electrode
This article is a report on a novel and high-stability biosensor with minor interference effects of surface modification of working electrode with β-Ga2O3 nanowires (NWs) on choline oxidase biosensor were investigated in an electrochemical detection system. β-Ga2O3 NWs were materialized on the silicon substrate in a catalyst-free growth mechanism. The β-Ga2O<sub...
متن کاملIn situ amplified chemiluminescent detection of DNA and immunoassay of IgG using special-shaped gold nanoparticles as label.
BACKGROUND Au(III) catalyzed luminol chemiluminescence (CL) is classic in luminescence analysis. Recently, spherical gold nanoparticles (Au-NPs) were found displaying far stronger catalytic activity on luminol CL than that of Au(III). Some methods based on Au-NPs probes have been developed for DNA detection or immunoassay. However, more complicated labeling or stripping procedures are often ine...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 50 93 شماره
صفحات -
تاریخ انتشار 2014